Analysis Note

ControlIncluded free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of protein with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

Research CategoryNeuroscience

Western blot: 1:200 using ECL on rat brain membranes.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Anti-Endothelin Receptor B Antibody detects level of Endothelin Receptor B & has been published & validated for use in IH & WB.

Research Sub CategoryCNS Control of MetabolismHormones & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified peptide corresponding to amino acids 298-314 of the rat Endothelin Receptor B (Accession P21451).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 µL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Specificity

Recognizes Endothelin Receptor B (ET-B). The immunogen sequence is specific for ET-B and is not present in any other known protein.

SPECIES REACTIVITIES: The immunogen sequence is identical in all known vertebrate proteins. Reactivity with other species has not been confirmed.

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.